Fermentation Group Lab

Fermentation Group Lab

Materials:

2-liter plastic soda bottle
Water
Brown Sugar
Blended Apple Flavorings
Yeast 

Procedure 

1. Clean and gather the equipment

2. In a pot, add ~1.75 liters of water, and all of the flavorings you plan to use. Bring the mixture up to a simmer, and let it bubble for 30 seconds. Turn off the heat and let the mixture cool.

3. In a separate bowl, dissolve 1.4 teaspoon of yeast into a small amount of warm water. When the yeast is dissolved, add it to the cooled soda base.

4. Pour the completed soda base into the 2-Liter bottle, and close the cap tightly. Let the bottle sit for 24 to 48 hours. The amount of time is variable, depending on the temperature of your kitchen, etc. At first, the carbon dioxide we be produced as a gas, and will increase the pressure in the bottle. Once enough pressure has been built up, newly produced carbon dioxide will begin to dissolve into the soda base.

This lab was an example of alcoholic fermentation, which produces ethanol. This is done by yeast; it can be found when it bubbled bubbles in soda. During alcoholic fermentation, the 2 pyruvate that comes from the glycolosis is converted into 2 acetaldehyde ( and gives off CO2), then uses 2 NADH to convert it into 2 ethanol