Friday, October 3, 2014

PCR

3 Advantages of PCR

PCR has three important advantages that that is important in the practice of forensics. One benefit from PCR is that you can produce more copies of a minimal starting amount in good for criminal investigation. In an investigation this is important because the extra copies help in identifying a suspect. Another advantage of PCR is you can reproduce a large amount of copies in a small amount of time. Lastly PCR can still be effective with small molecular weight DNA samples. Even if the same is damaged in many ways, PCR can still function properly. RFLP requires higher molecular weight. There are 12 steps tp RFLP. 1 is visual evaluation, 2 is assay, 3 is digestion, 4 is test gel, 5 is known sample perpartion, 6 is anaytical electrophoresis, 7 is southern blot, 8 is hybridizing, 9 is autoradiography, 10 is imaging, 11 is striporing and 12 is more probing. PCR is a more common practice than RFLP beacuse in most crime scences, only a small DNA sample is avaliable.

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